|
KMID : 0364820160520030298
|
|
Korean Journal of Microbiology
2016 Volume.52 No. 3 p.298 ~ p.305
|
|
|
Identification of three pathways for p-cresol catabolism and their gene expression in Pseudomonas alkylphenolica KL28
|
|
Sung Jin-Il
Lee Kyoung
|
|
|
Abstract
|
|
|
|
Previously our laboratory showed that Pseudomonas alkylphenolica KL28 possesses two different lap and pcu gene clusters for p-cresol catabolism. In this study, additional gene cluster (pchACXF-pcaHG-orf4-pcaBC) has been identified to encode enzymes necessary for catabolism of p-cresol to β-carboxy-cis,cis-muconate. This gene cluster showed almost identical nucleotide sequence homologies to those in the plasmid of Pseudomonas putida NCIMB 9866 and 9869, British origins, indicating the possibility of a horizontal gene transfer. Through mutagenesis of each gene cluster and gfp-based promoter reporter assays, it has been shown that the three gene clusters are functionally operated and pch genes are induced by p-cresol. Furthermore, the pcu gene cluster of the three was shown to be dominantly expressed in utilization of p-cresol. Mutation of the pcu gene was defective in aerial structure formation under p-cresol vapor, indicating the utilization rate of carbon source is one of key elements for the multicellular development of this strain.
|
|
|
KEYWORD
|
|
|
Pseudomonas alkylphenolica, catabolism, p-creso, , isoenzyme
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|